ZOE Fluorescent Cell Imager

描述

The ZOE Fluorescent Cell Imager eliminates the complexities of cell imaging associated with traditional microscopes. This fluorescence imaging system combines the ease of use of a personal tablet with the power of an inverted microscope.

An Android-based platform, the ZOE Cell Imager uses an intuitive touch-screen interface to control the brightfield, three fluorescence channels, and the integrated digital camera.

The ZOE Fluorescent Cell Imager is a complete digital imaging system, allowing users to view samples, capture and store images, and create multicolor overlays. Thanks to the built-in light shield, the ZOE Cell Imager does not require a darkroom for fluorescence imaging.

Features and Benefits of the ZOE Fluorescent Cell Imager

• Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training
• Flexible operation — brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications
• Fluorescence imaging at your bench — light shield permits fluorescence imaging in ambient light
• Robust construction — fully integrated system with long-life LEDs, ready for intensive daily use
• LED light sources — thousands of hours of illumination that are instantly ready after power-on
• Large viewing area — the motorized stage and wide field of view allow you to see more of your sample, faster
• Small footprint — compact size accommodates crowded lab benches

Applications of the ZOE Fluorescent Cell Imager

Use the ZOE Cell Imager to check/screen samples prior to high-content analysis (HCA), high-throughput screening (HCS), confocal imaging, or fluorescence-activated cell sorting (FACS). With a brightfield and three fluorescent channels, the ZOE Cell Imager has all the features needed for daily cell culture work as well as fluorescent applications:

• Visual estimation of cell confluency
• Observation of general cell health and morphology
• Cell growth and proliferation monitoring
• Capturing cell images (with or without fluorescent labels)
• Visualization of expressed fluorescent proteins
• Immunofluorescent protein localization
• Estimation of transfection efficiency

Illumination Light Sources

• Blue channel uses a UV LED
• Green channel uses a blue LED
• Red channel uses a green LED
• Brightfield channel uses a ring of multiple green LEDs for reduced chromatic aberration

Fluorophores Compatible with the ZOE Fluorescent Cell Imaging System

This is not a comprehensive list; other dyes and fluorescent proteins with compatible excitation and emission spectra can also be used.

Blue Channel Excitation: 355/40 nm Emission: 433/36 nm	Green Channel Excitation: 480/17 nm Emission: 517/23 nm	Red Channel Excitation: 556/20 nm Emission: 615/61 nm
PureBlu DAPI Nuclear Staining Dye	CytoTrack Green 511/525	ReadiLink 555/570 Antibody Labeling Kit
PureBlu Hoechst 33342 Nuclear Staining Dye	ReadiLink 492/516 Antibody Labeling Kit	ReadiLink 594/610 Antibody Labeling Kit
ReadiLink 350/440 Antibody Labeling Kit	VivaFix 498/521 Cell Viability Assay*	VivaFix 547/573 Cell Viability Assay
VivaFix 353/442 Cell Viability Assay	CFDA-SE	VivaFix 583/603 Cell Viability Assay
Alexa Fluor 350	Acridine Orange	Alexa Fluor 546
Alexa Fluor 405	Alexa Fluor 488 dye*	Alexa Fluor 568
Cascade Blue	BODIPY Fl*	Alexa Fluor 594
CellTracker Blue	Calcein AM	Alexa Fluor 610
DAPI*	DiO	Cy3*
Hoechst*	EGFP*	Dil Stain
LysoTracker Blue	ER-Tracker Green	DsRed*
Marina Blue	FITC*	ER-Tracker Red
NucBlue Fixed	MitoTracker Green FM	mCherry*
NucBlue Live	SYTO 9, SYTO 13, SYTO 16	mStrawberry
—	SYTOX Green	MitoTracker Red*
—	Tubulin Green	mKate*
—	YFP*	RFP*
—	—	SYTOX Orange
—	—	SYTO 84, SYTO 85
—	—	Texas Red*

* R&D Tested

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技术指标

Imaging channels	Brightfield channel and 3 fluorescence channels (blue, green, and red)
Light source	Blue channel: UV LED Green channel: blue LED Red channel: green LED Brightfield channel: multiple green LEDs (reduces chromatic aberration)
User interface	10.1 in. color (26 cm) touch-screen LCD monitor, with anti-glare and anti-fingerprint treatment, 1,280 x 768 pixel image resolution, 80–180° angle tilt range
Focusing mechanism	Coarse and fine, manual adjustment
Camera	Monochrome camera, 12 bit CMOS, 5 megapixels
Data format	JPEG, TIFF, or RAW image files
Image merge	Images from up to 4 channels can be overlaid
Data storage	16 GB internal memory (~2,500 JPEG files,1,500 TIFF files, 400–800 RAW files)
Data export	Yes, 2 USB ports
Display output	Yes, 1 HDMI port
Objective	20x
Numerical aperture	0.40
Display magnification	Standard: 175x; zoom: 700x
Maximum imaging area	0.70 mm2 field of view
Motorized stage	6 mm travel in X, Y direction, touch-screen control of travel speed and direction
Compatible with	Flasks: T25, T75, or T225 Multiwell plates: 6-, 12-, 24-, 48-, 96-, or 384-well microplates Dishes: 35 mm, 60 mm, or 100 mm Slides: chamber slides or standard glass microscopy slides
Software	Stand-alone Android operating system; PC is not required for operation
Instrument size (L x W x H)	33 x 32 x 30 cm (13 x 12.6 x 11.6 in.)
Instrument weight	9 kg (19.7 lb)