ChemiDoc MP Imaging System

描述

The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.

Features and Benefits

• All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
• Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright Fluorophores; see the fluorophore compatibility chart
• Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
• Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
• Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
• Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
• Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Performing Stain-Free western blotting using the ChemiDoc MP System provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Applications and Uses

• Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
• Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection

Included Components

• Internal computer with Image Lab Touch Software
• 12” touch screen display
• Image Lab Software to install on your computer
• Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi ChemiDoc MP Stain-Free Western Blotting also include:

• ChemiDoc MP Imaging System
• Mini-PROTEAN Tetra Cell, 4-gel (1658004) or Criterion Cell (1656001) Electrophoresis Chamber
• PowerPac Basic Power Supply (1645050)
• Trans-Blot Turbo Transfer System (1704150)
• 4–20% Mini-PROTEAN TGX Stain-Free or 4–20% Criterion TGX Stain-Free Protein Gels
• Buffers, Nitrocellulose Transfer Packs, Immun-Blot LF PVDF Membranes, and other consumables

Optional Components

• White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
• Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12003027)
• Holder for ChemiDoc Sample Trays and UV Shield (1708377)
• Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
• UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (>12003914)

Configuration Options

Product	Catalog Number
ChemiDoc MP Imaging System	17001402
ChemiDoc MP Stain-Free Western Blotting Workflow for Mini Gels	17001395
ChemiDoc MP Stain-Free Western Blotting Workflow for Midi Gels	17001389

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Note: Also available are the ChemiDoc Imaging System (17001401), ChemiDoc Stain-Free Western Blotting Workflows for Mini Gels (17001403), and ChemiDoc Stain-Free Western Blotting Workflows for Midi Gels (17001390), which have a more limited fluorescence capability than ChemiDoc MP Systems. These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).

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技术指标

Automation Capabilities
Smart Tray Technology™	Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus	Precalibrated focus for any zoom setting or sample height
Auto-exposure	Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding	Dynamic; precalibrated and optimized for each application
Hardware
Touch-screen functionality	Multitouch capability (2 points) 12.1" display
Maximum image area (W x H)	21 x 16.8 cm
Detector	Cooled CCD, 6 megapixels
Dynamic range	>4 orders of magnitude
Illumination sources	Trans-UV, 302 nm excitation Epi-white Trans-white (requires White Sample Tray) Trans-blue, 450−490 nm excitation (requires Blue Sample Tray) Epi-blue, 460−490 nm excitation Epi-green, 520−545 nm excitation Epi-red, 625−650 nm excitation Epi-far red, 650−675 nm excitation Epi-near IR, 755−777 nm excitation
Filter and application	590/110 nm standard filter to perform protein and DNA gel and blot imaging Chemiluminescence filter 518–546 nm filter for blue-excitable fluorophores and stains 577–613 nm filter for green-excitable fluorophores and stains 675–725 nm for red-excitable fluorophores and stains 700–730 nm filter for far red-excitable fluorophores and stains 813–860 nm for near IR-excitable fluorophores and stains
Data output	16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight	35 kg (78 lb)
Instrument size (L x W x H)	61 x 51 x 53 cm
Operating voltage	100–250 V
Operating temperature	10–28°C
Operating humidity	10–85% relative humidity (non-condensing)